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Figure 9


Fig. 9. Kinetics of endosome-to-TGN retrieval of CI-MPR and sortilin is perturbed in PIKfyve-suppressed cells. (A,C) Images depicting the kinetics of delivery of a cell-surface-labelled (A) CD8-CI-MPR chimera or (C) CD8-sortillin chimera (both in green) to the TGN46-labelled TGN (red) in control HeLaM cells or in HeLaM cells whose PIKfyve was suppressed by a combination of duplex II and V. Images are projections of eight confocal sections and are representative of more than 40 cells images for each condition. Bars, 20 µm. (B,D) Quantification of the kinetics of cell-surface-labelled (B) CD8-CI-MPR chimera or (D) CD8-sortilin chimera transport to the TGN46-labelled TGN in HeLaM cells, expressed as percent normalised colocalisation between the two labels. Data were obtained as outlined in Materials and Methods from n>=40 cells for each time point, and are expressed as the mean ± s.e.m.





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