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Figure 1


Fig. 1. Apaf-1-/- MEFs show resistance to ER stress-induced cell death. (A) Cell survival of Apaf-1+/- (WT) and Apaf-1-/- (KO) MEFs in response to ER stress. MEFs were treated with the indicated doses of tunicamycin or thapsigargin for 24 hours and cell survival was determined by Annexin V and propidium iodide (PI) staining with flow cytometry: Annexin V and PI negative population was regarded as viable. Shown are mean + s.d. (B) caspase-3 activation during ER stress. Apaf-1+/- and Apaf-1-/- MEFs treated for indicated hours with 1 µg/ml tunicamycin or 0.1 µM thapsigargin were lysed and analyzed for caspase-3 cleavage. (C) Time course of ER stress-induced cell death of MEFs. MEFs were treated with either 1 µg/ml tunicamycin or 0.1 µM thapsigargin for indicated hours, collected, and analyzed for cell viability as described in A. Shown are mean ± s.d. (D) Transmission electron microscopic analysis of Apaf-1+/- and Apaf-1-/- MEFs. MEFs were treated with 1 µg/ml of tunicamycin for 0, 48 or 72 hours. Experiments were repeated three times with similar results.





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