spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Caspase-12 is processed downstream of the apoptosome in MEFs in response to ER stress. (A) Caspase-12 processing during ER stress-induced cell death was analyzed by western blotting. MEFs (Apaf-1+/-; WT or Apaf-1-/-; KO) were treated with tunicamycin (1 µg/ml) with or without Caspase-3 specific inhibitor Ac-DNLD-CHO (100 mM) for the indicated time. Caspase-12 (C12) activation was detected by western blotting. C3, caspase-3. (B) MEFs were introduced with wild or mutated caspase-12 and analyzed for caspase-12 processing by western blotting. (C) Effect of caspase-12 overexpression to ER stress-induced cell death was analyzed by flow cytometry (Annexin V and PI staining). (D) Effect of caspase-12 overexpression on caspase-3 activation was determined by western blotting. (E) Reduced expression of caspase-12 by siRNA was confirmed by western blotting. (F) Apaf-1+/- (WT) or Apaf-1-/- (KO) MEFs with reduced caspase-12 expression (by C12 si-3 in E) were analyzed for their cell survival by flow cytometry. Shown are means ± s.d. Experiments were repeated twice with similar results.





Right arrow Return to article