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Figure 6


Fig. 6. SNX5 associates with discrete lipid microdomains on the surface of macropinosomes. (A) HEK-GFP-SNX5 cell monolayers were fixed in 0.9% PFA and analysed by indirect immunofluorescence using a monoclonal antibody against EEA1 followed by Cy3-conjugated goat anti-mouse IgG. (B) Surface rendered 3D reconstruction of the same macropinosome from A was generated from Z stacks using a LSM 510 META confocal laser-scanning microscope. Presented is a reconstruction rotated 45° on the y-axis. Optical sections used were 0.8 µm in depth.





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