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Figure 7


Fig. 7. SNX5 associates with early-stage macropinosomes. HEK-GFP-SNX5 and HEK293 cell monolayers were transfected as described in the Materials and Methods, serum starved overnight and exposed to 100 ng/ml EGF for 5 minutes before being fixed with 0.9% PFA. SNX5-positive macropinosomes were then analysed by indirect immunofluorescence. Images were collected as described in Fig. 1B. (A-C) HEK-GFP-SNX5 cell monolayers were labelled with monoclonal antibodies against SNX1, EEA1 and LAMP1, respectively, followed by Cy3-conjugated goat anti-mouse IgG secondary antibody. (D-F) HEK293 cell monolayers co-transfected with pCMU-myc-SNX5 and either GFP-Rab5wt (C), GFP-Rab7wt (D) or YFP-Rab11wt (E) as indicated were co-labelled with an anti-myc monoclonal antibody and Cy3-conjugated goat anti-mouse IgG. Bar, 5 µm.





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