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Figure 8


Fig. 8. SNX5-positive tubule departure is concurrent with macropinosome Rab5 to Rab7 conversion. (A,B) HEK293 cell monolayers were co-transfected with mCherry-SNX5 and either GFP-Rab5wt (A) or GFP-Rab7wt (B). Time-lapse movies were recorded with an interval between image capture of 2 seconds. Bars, 5 µm. (C) HEK-GFP-SNX5 cells and HEK293 cell monolayers transfected with either GFP-Rab5wt or GFP-Rab7wt were serum starved overnight before being incubated with dextran-TR and 100 ng/ml EGF for 3 minutes at 37°C before fixation with 0.9% PFA at 4°C. 50 dextran-positive macropinosomes (diameter >0.2 µm) were imaged and the presence of each marker was scored. Presented is the percentage of dextran-positive macropinosomes labelled with detectable levels of GFP-Rab5, GFP-SNX5 or GFP-Rab7.





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