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Figure 2


Fig. 2. Expression of intermediate filaments and ECM constituents in the sciatic nerve of GFAP-null mice. (A-C) Double staining for L1 and vimentin (merge in C) shows vimentin expression in non-myelin-forming Schwann cells. (D-F) Double-staining for MAG and vimentin (merge in F) shows vimentin expression in myelin forming Schwann cells. (G) Western blot analysis shows comparable levels of nestin and vimentin in mutants compared with wild type; ß-tubulin was used to normalize the samples. (H,I) Fibronectin expression in sciatic nerves of wild-type and GFAP-null mice; an increased amount of fibronectin was observed in the endoneurium in mutants. (J,K) Collagen IV expression in sciatic nerves of wild-type and GFAP-null mice. Mutants and controls showed comparable levels of collagen IV. (L) Western blot analysis confirmed an increased amount of fibronectin in the sciatic nerve of GFAP-null mice compared with controls, whereas collagen IV and laminins were present at equal amounts. SDS-PAGE gels were 7.5% except when analyzing laminins (5%). Bar in L, 60 µm for A-C; 20 µm for D-F and H-K.





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