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Figure 5


Fig. 5. Vimentin upregulation is maintained in nerves of GFAP-null mice after sciatic nerve injury. (A-H) Longitudinal sections of 3-day-old injured sciatic nerves from wild-type (A,C,E,G) and GFAP-null mice (B,D,F,H) double-stained for vimentin and neurofilaments (A,B), NCAM and neurofilaments (C,D), L1 and neurofilaments (E,F), p75NTR and neurofilaments (G,H). DAPI staining of nuclei (blue). The dedifferentiated Schwann cells showed expression of the above molecules as in wild type. Bar, 30 µm. (I,J) Protein extracts from the distal stump of crushed nerves from wild-type (I) and GFAP-null mice (J) at different time points were immunoblotted with an anti-vimentin antibody. Sample loading was normalized against ß-tubulin. Ratio of vimentin to ß-tubulin was measured by densitometry and expressed in the bottom line as times of increase at each time point T (in days) relative to T zero. Compared with control mice, vimentin is similarly upregulated in nerves of GFAP-null mice.





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