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Figure 1


Fig. 1. Vps1p, Dnm1p and Fis1p play a role in regulating peroxisome numbers in S. cerevisiae. Quantitative data on peroxisome abundance were obtained by fluorescence microscopy of GFP-SKL producing S. cerevisiae strains cultured on glucose (A,C,E,G,I) or in the presence of oleate (B,D,F,H,J). The number of fluorescent spots per cell was counted from randomly taken fluorescence microscope images. For each sample, fluorescent spots were counted in 300 non-budding cells taken from two independent cultures (150 cells per culture). The frequency distributions of cells with different numbers of fluorescent spots are shown. Error bars represent the s.e.m.





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