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Fig. 2. Peroxisome and mitochondrial morphology in oleate-induced cells. (A) Representative fluorescence images of GFP-SKL-producing, oleate-induced cells of WT and the various mutant strains. In dnm1 and vps1 cells the peroxisome number is reduced. In vps1 and dnm1 vps1 cells large peroxisomes are present, which are often constricted. (B) Examples of the mitochondrial morphology in oleate-induced cells of the same strains. Mitochondria were stained using MitoTracker Orange. In WT and vps1 cells a branched mitochondrial network is evident. In dnm1, fis1 and dnm1 vps1 cells generally one long tubular structure is present that contains fewer branches than in WT cells and remains near the cell cortex. (C) A GFP-Ant1p-producing dnm1 vps1 cell induced on oleate medium. GFP-Ant1p is localized to peroxisomal membranes. As in the dnm1 vps1 cells producing GFP-SKL, generally a single, large peroxisome is observed per cell. Bars, 5 µm.
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