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Fig. 4. Peroxisome dynamics in S. cerevisiae WT and mutant strains. Selected images (a-e) taken from time-lapse videos of WT (lane A), vps1 (lane B) and dnm1 vps1 cells (lane C), grown in the presence of oleate. The corresponding Movies 1-3 are shown in the supplementary material. In each panel the fate of two cells that resulted from budding is depicted. The data show that in WT cells (A) peroxisomes developed in buds at the early stage of development. In vps1 cells (B) low numbers of peroxisomes are present. Developing buds may be administrated by peroxisomes in two different ways. In the cell on the right-hand side, a small peroxisome had initially migrated into the bud, followed by a second organelle that arose from the larger organelle located in the neck region. In the cell on the left-hand side, a single organelle is initially observed that migrates to the neck, elongates and subsequently divides at a late stage of budding. A similar elongation effect is observed during the second budding of the cells on the right-hand side, confirming that administration of peroxisomes to developing buds may occur by two different mechanisms. In cells of the dnm1 vps1 double mutant (C) only one mode of segregation is observed. In these cells the single peroxisome structure migrates to the neck between the mother cell and the bud, elongates into the bud and is separated at a very late stage of bud development, probably during cytokinesis. Bars, 5 µm.
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