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Figure 6


Fig. 6. Dnm1-GFP co-localizes with mitochondria and peroxisomes. (A) The location of Dnm1-GFP to mitochondria visualized by MitoTracker Orange. Note that all GFP spots, except for one (upper right), co-localize with mitochondria. Cells were grown on glucose media. (B) A section through a cell in which two peroxisomes are observed, labelled with DsRed-SKL. One of these shows co-localization with Dnm1-GFP (arrow), the remaining Dnm1-GFP spots are considered to localize to mitochondria (compare with A). The images represent a selected single section through a Z-stack, prepared by CLSM (see supplementary material Movie 5). Cells were grown on glucose. The inset shows a magnification of the organelle indicated by the arrow. (C) Location of Dnm1-GFP is shown in glucose-grown fis1 cells. In these cells the number of Dnm1-GFP spots is strongly reduced (compare with A,B). These spots do not co-localize with peroxisomes, which are labeled with DsRed-SKL. (D) Location of GFP-Fis1p. Most of the GFP is present on large structures, which represent mitochondria. A minor portion of the GFP-Fis1p fluorescence is present in small spots, which co-localize with the peroxisomal marker protein DsRed-SKL. Bars, 5 µm.





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