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Figure 1


Fig. 1. Intracellular distribution of Kank protein and locations of potential NLS and NES motifs. (A) Intracellular distribution of Kank protein. The localization of endogenous Kank protein in OS-RC-2 (upper) and VMRC-RCW (lower) cells was examined by immunostaining with an FITC-conjugated antibody against Kank protein. The nuclei were stained with DAPI. LM, light microscopy. Bar, 20 µm. (B) The sequences of NLS (NLS1 and NLS2) and NES (NES1 to NES3) motifs predicted in Kank-L. The conserved sites are underlined (NLSs) or shadowed (NESs), which were substituted by alanines in the respective mutants. (C) The maps of Kank-L and Kank-S.





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