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Figure 3


Fig. 3. Identification of NESs. (A) Schematic structures of FLAG-tagged GST (FLAG-GST) or GST constructs of FLAG-tagged N-terminal Kank with wild-type (Nt-WT-GST), or mutant NES1 (NES1m-GST) or mutant NES2 (NES2m-GST). (B) Representative images of NIH3T3 cells transiently transfected with the fusion constructs shown in (A). The localization of FLAG-tagged GST fused protein was monitored by immunostaining with an FITC-conjugated antibody and the nucleus was stained with DAPI. LM, light microscopy. (C) Quantification of the cells according to the localization of GST-fused proteins. (D) Schematic structures of GST constructs of FLAG-tagged partial Kank without NESs (NESnull-GST) or with NES3 (NESnull-GST-NES3). In NESnull-GST, the first NES was deleted and the second was mutated. (E) Representative images of NIH3T3 cells transiently transfected with the fusion constructs shown in (D). The localization of FLAG-tagged GST fused protein was monitored by immunostaining with an FITC-conjugated antibody and the nucleus was stained with DAPI. (F) Quantification of the cells according to the localization of GST-fused proteins. Bar, 20 µm.





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