spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Localization of full length Kank-L and its mutants. (A) Schematic structures of FLAG-tagged full-length Kank-L constructs with wild-type NLS and NES motifs (Kank-L-WT), or with mutant NLS (NLSm) or NES (NESm) motifs. (B) Representative images of NIH3T3 cells transiently transfected with the fusion constructs shown in (A). The cells transfected with NLSm were treated with or without leptomycin B. The localization of FLAG-tagged GST fused protein was monitored by immunostaining with an FITC-conjugated antibody and the nucleus was stained with DAPI. LM, light microscopy. (C) Quantification of the cells according to the localization of GST-fused proteins. Bar, 20 µm.





Right arrow Return to article