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Figure 5


Fig. 5. The effect of leptomycin B on the localization of Kank protein. (A) Representative images of OS-RC-2 cells showing endogenous Kank protein detected with anti-Kank antibody before or after treatment with leptomycin B (LMB). LMB-, the cells without LMB treatment; LMB+, the cells treated with LMB for 8 hours. (B) Representative images of NIH3T3 cells transiently transfected with the constructs of Kank-L or Kank-S and the nucleus was stained with DAPI. (C) Quantification of the cells according to the localization of FLAG-tagged Kank-L protein after treatment with LMB for the periods indicated. (D) Quantification of the cells according to the localization of FLAG-tagged Kank-S protein after treatment with LMB for the periods indicated. The results in (C) and (D) were scored from triplicate transfections. Bar, 20 µm.





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