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Figure 2


Fig. 2. Transient upregulation of p110{alpha} and p110ß mRNAs during in vitro differentiation of human keratinocytes. (A) Samples of 20 µg total RNA from adult human keratinocytes grown in primary culture under differentiation-promoting conditions were analyzed by northern blotting using cDNA probes specific for p110{alpha} and p110ß. ß-actin or involucrin probes were hybridized as a loading control or to monitor differentiation, respectively. (B) Western blot analysis verifies transient upregulation of p110{alpha} protein upon differentiation. The blot was re-incubated with antibodies against phosphorylated Akt (Ser473), PTEN, involucrin, keratin 10 (K10), and ß-actin to verify upregulation of differentiation markers (involucrin, keratin 10) or as a loading control (ß-actin).





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