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Figure 4


Fig. 4. Validation of PI3K target genes. (A) Western blotting reveals upregulation of RTP801 following PI3K-activation. Unaltered RTP801 levels were seen in vector-transfected control clones. Equal loading was verified by treatment of the membranes with a ß-actin antibody. (B) RNAs isolated from independent Myr-p110{alpha}*-mER expressing or vector-transfected cell clones were subjected to real-time RT-PCR. Genes encoding {alpha}-thalassemia/mental retardation syndrome X-linked protein (ATRX), periphilin1 protein (PPHL1), ets-homologous factor 3 protein (EHF) and Cockayne syndrome 1 protein (CKN1) were tested for differential expression in 4-OHT-treated Myr-p110{alpha}*-mER expressing cell clones. Values are mean ± s.d. The table summarizes the average fold regulation observed by real-time RT-PCR and microarray analysis normalized to endogenous controls and relative to 5 hour solvent controls. Solvent treatment is indicated by -, 4-OHT treatment by +.





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