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Figure 7


Fig. 7. Activation of the PI3K pathway in HaCaT keratinocytes delays differentiation. (A) Western blotting for detection of involucrin during suspension-culture-induced differentiation of Myr-p110{alpha}*-mER HaCaT cell clones treated with 4-OHT or solvent, respectively. Western blot detecting phosphorylated Akt (Ser473) indicates successful stimulation of PI3K-signaling by 4-OHT-treatment. Incubation of the membrane with an antibody to ß-actin was used as a loading control. The time of cultivation in suspension is indicated on top of the lanes. (B) Cells grown in serum-free medium over 11 days were harvested after the time points indicated, and the corresponding protein lysates were subjected to western blot analysis for involucrin and phosphorylated Akt (Ser473). Staining of the membrane with an antibody against ß-actin served as a loading control.





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