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Figure 8


Fig. 8. Myr-p110{alpha}*-mER cell clones form a hyperthickened epithelium in organotypic cultures. (A) hematoxylin and eosin and BrdU stainings of solvent-treated, 4-OHT-treated or untreated organotypic cultures of the two independent Myr-p110{alpha}*-mER expressing cell clones 1 (a-f) and 17 (g-m) and a vector-transfected cell clone (n-s) are shown. Arrowheads in b indicate disorganized segments of the basal layer, where keratinocytes protrude into the collagen matrix. Arrowheads in e and 1 indicate suprabasal BrdU-positive cells. Bars, 50 µm. (B) Epithelial thickness of the cultures. (C) Bar graph representing the number of BrdU-positive cells per mm basement membrane. Error bars indicate s.e.m. ***P<0.001; ns, not significant.





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