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Fig. 9. Delayed differentiation of keratinocytes expressing Myr-p110^*-mER in organotypic cultures. (A) 4-OHT-treated and solvent-treated (control) organotypic cultures of Myr-p110^*-mER expressing HaCaT cells were analyzed for phosphorylated Akt (a,b, green), K14 (c,d, green), K10 (e,f, green), involucrin (g,h, blue), loricrin (l,m, green) and 6 integrin (c,d,i,k, red) by immunofluorescence. Arrowheads in d indicate strong K14 expression in the upper suprabasal layers. Nuclei were stained with Hoechst 33342 and are shown in blue in e and f. Note the apical 6 integrin expression in suprabasal cells (k, arrowheads), which is not observed in the controls (i). Counterstaining in l and m was performed with propidium iodide (red). (B) Paraffin sections of 4-OHT-(c,d) or solvent-treated (a,b) Myr-p110^*-mER expressing organotypic cultures were subjected to immunofluorescence staining using an antibody recognizing p63. Nuclei are counterstained in a and c with Hoechst 33342. The basement membrane is indicated with a dotted line. Bars, 50 µm.

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