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Figure 10


Fig. 10. The DG extracellular domain alone is crucial to laminin assembly. (A) Western blot of cell extracts prepared from a DG-/- MEpL cell line (entirely DG-/-) infected with retroviral vector (VEC) or that encoding full-length DG (wtDG), a fusion protein comprised of the extracellular DG sequences fused to the transmembrane region of TACE (DG-tmf), or deletions within the {alpha}-DG mucin domain (DEL D and E). Blots were incubated with antibodies specific for {alpha}-DG, N-terminal ß-DG, or E-cadherin (loading control). Sizes of molecular mass markers are shown in kDa. (B) Immunofluorescent images of cells in A, treated for 4 hours with 10 nM exogenously added laminin-111-FITC. Corresponding phase images are shown in the bottom panel. Bar, 10 µm.





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