|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Partial-DG+/+ colonies grown in a 3D matrix of collagen-I-laminin-111 retain laminin and DG colocalization on the surface of DG+/+ cells only, but fail to polarize. (A,B) Confocal immunofluorescent images taken at the center of partial-DG+/+ MEpG colonies grown in collagen-I-laminin-111 and co-immunostained as follows: (A) 
6 integrin and C-terminal ß-DG antibodies were detected using Rhodamine-(red) and Cy5-(blue) labeled secondary antibodies, respectively. Actin was visualized with Alexa Fluor-488-phalloidin (green). (B) Laminin, 6 integrin and C-terminal ß-DG antibodies were detected using Rhodamine-(red), FITC-(green), and Cy5-(blue) labeled secondary antibodies, respectively. Arrows show part of colony surface lacking laminin and ß-DG staining. (C) Confocal immunofluorescent images were taken at the cell surface of the colony shown in B to reveal co-staining for laminin and ß-DG, and their extent of co-localization. Dotted outline represents outer edge of colony. Bar, 10 µm.
|
