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Fig. 6. Loss of ß-casein production in response to laminin-111 in DG^-/- cells. (A) Western blot of cell extracts (10 µg protein) prepared from DG^+/+ and partial-DG^-/- MEpL cell populations incubated with laminin-111 overlay in the absence (-) or presence (+) of prolactin and hydrocortisone. Blots were incubated with antibodies specific for ß-casein or E-cadherin (loading control), followed by HRP-conjugated secondary antibodies. Sizes of molecular mass markers are shown in kDa. Dotted lines separate non-adjacent lanes derived from the same blot. (B) Immunofluorescent images of DG^+/+ and partial-DG^-/- cell population treated with 10 nM exogenously added laminin-111-FITC for 4 hours. Samples were co-stained using C-terminal ß-DG antibody and Rhodamine-labeled secondary antibody. Corresponding phase images are shown in the bottom panel. Arrows point to a DG^-/- cell lacking laminin-111-FITC binding.

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