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Figure 6


Fig. 6. Subcellular localisation of MARK3. (A) Wild-type (wt) or indicated mutant forms of GST-MARK3 (see Fig. 5) were expressed in HEK 293 cells, which were fractionated into a cytosolic and a membrane fraction. Subcellular fractions, including solubilised portions of the homogenates, were subsequently analysed by western blot with regards to MARK3 ({alpha}GST), MARK3 T-loop phosphorylation ({alpha}p-T211), the cytosolic marker GAPDH ({alpha}GAPDH) and the plasma membrane marker Na-K-ATPase ({alpha}Na-K-ATPase). The results are representative of three experiments. (B) Wild-type (wt) or indicated mutant forms of GFP-MARK3 (see Fig. 5) were expressed in HEK 293 cells, which were fixed in 3% paraformaldehyde 24 hours post transfection. The GFP-fluorescence was analysed by confocal fluorescence microscopy. The cells shown are representative of images obtained in two separate experiments. Bars, 10 µm.





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