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Figure 1


Fig. 1. Overview of the organisation of clathrin and of the constructs used in this study. (A) Model of a clathrin triskelion proposed by Fotin et al. (Fotin et al., 2004). The triskelion is viewed looking down onto the vertex. Coloured regions show the features of a CHC molecule (see key, right). (B) Schematic representations of each CHC construct used in the study. Variable region (residues 1631-1675) is shown in grey, GFP has been omitted for clarity. Short names used in the paper are in black and full descriptive names are in grey. Trimerisation was predicted based on previous publications (Fotin et al., 2004; Liu et al., 1995; Nathke et al., 1992; Ybe et al., 2003). X22 epitope is between residues 1109-1128 of CHC (Liu et al., 1995). Constructs were compared with `GFP', GFP expressed on a CHC RNAi background and with `Control', GFP expressed on a control RNAi background.





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