spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Stunted is a trimeric molecule. (A) Sedimentation analysis of 1-1639 and 1-1597. Quantification of a typical experiment where cell lysates were separated on a 15-40% glycerol gradient. Note the second peak at fraction 8 in 1-1639 that is absent in 1-1597. (B) Sedimentation analysis of Stunted and Stunted{Delta}tripod. Quantification of a typical experiment where cell lysates were separated on a 10-35% glycerol gradient. Note the presence of a second peak in Stunted that is absent in Stunted{Delta}tripod. The sedimentation of molecular mass standards is shown for each gradient (arrows). The calculated molecular masses for monomers of 1-1639, 1-1597, Stunted and Stunted{Delta}tripod were 215, 210, 117 and 108 kDa respectively. Fractions were analysed by SDS-PAGE and immunoblotting for GFP. Following densitometry, the signal in each fraction was expressed as a percentage of the total GFP signal from all fractions on the blot.





Right arrow Return to article