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Figure 5


Fig. 5. Constructs containing the N-terminal domain were recruited to the mitotic spindle. (A) Representative confocal micrographs of each construct in HEK293 cells in metaphase. Bar, 10 µm. (B) A cell expressing 1-1639 on a knockdown background. ROIs (grey, 1.04x1.04 µm) were placed over the spindle (black outline) and in the cytoplasm (white outline). Spindle regions and non-spindle areas were defined by staining for {alpha}-tubulin using DM1A/Alexa Fluor 546 (left). Measurements were taken from the GFP channel (right). (C) Histogram to show the recruitment to the spindle of CHC constructs on a CHC-knockdown background. Spindle recruitment is the GFP fluorescence measured in a 1.04x1.04 µm ROI at the spindle divided by that measured in a same-sized ROI in the cytoplasm (Fspindle/Fcytoplasm). Results are mean ± s.e.m. of 12-27 cells per construct; **P<0.01 compared with levels in GFP cells.





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