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Figure 1


Fig. 1. Phagosome formation in cells expressing the CRAC-GFP protein. Cells expressing CRAC-GFP (green) were allowed to engulf labeled yeast (red) for 0, 1, 2, 3, 5 or 10 minutes. The cells were then fixed and processed for immunofluorescence analysis. (A) Representative examples of a phagocytic cup, of a newly formed phagosome and of a maturing early phagosome. A white line indicates the delimitation of each cell. Bar, 4 µm. (B) The graph indicates the percentage of each structure compared with the total number of CRAC-GFP positive cells. At each time point, at least 60 cells engaged in phagocytosis and expressing CRAC-GFP were considered. The experiment was repeated two times with identical results. After 1 minute, phagocytic cups (triangle) were visible. After 2 minutes, CRAC-GFP-positive newly formed phagosomes (square) were detectable while maturing early phagosomes without CRAC-GFP staining (circle) were observed after 3 minutes.





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