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Figure 2


Fig. 2. (A) The ifta-2 promoter fusion to GFP shows expression specifically in ciliated sensory neurons. Data for amphid and labial neurons in the head are shown. Expression is also present in the ciliated phasmid neurons in the tail (data not shown). In panels A-D, the head of the worm is towards the left and in B and D, arrows indicate cilia localization. (B) IFTA-2::GFP translational fusion protein localizes to the base of cilia and within the cilia axoneme in the head and tail (not shown) of the adult hermaphrodite. (C) A single point mutation (T42N) in the GTP binding domain of IFTA-2::GFP causes diffuse localization of the fusion protein throughout the ciliated sensory neurons with no cilia localization. (D) A single point mutation (D123L) in the G3 nucleotide binding domain of IFTA-2::GFP does not disrupt cilia localization of the fusion protein. (E) Immunofluorescence localization of the RabL5 protein in inner medullary collecting duct cells derived from the mouse kidney, detected with an antibody against mammalian RabL5 (green) and acetylated tubulin (red). Both proteins colocalize to the primary cilia. RabL5 is also concentrated at the basal body at the base of cilia. Inset shows RabL5 localization alone.





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