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Figure 2


Fig. 2. Ligands regulate large-scale chromatin structure. (A-D) GFP-ER was transiently expressed in PRL-HeLa cells, and then treated with (A) ethanol, (B) E2, (C) 4HT or (D) ICI for 2 hours prior to fixation. Decondensed arrays are seen in vehicle- and E2-treated cells, although E2 treatment results in further decondensation, and condensed arrays are seen in 4HT- and ICI-treated cells. (E) Cells transiently expressing GFP-ER were treated with either ethanol (ETOH, vehicle) or 10 nM of ligand (E2, 4HT, ICI) for 2 hours. After fixing and counter-staining with DAPI, cells were imaged and array size was quantified using HTM as described in Materials and Methods. Note that array size correlates positively with transcription signal obtained by FISH (Fig. 3). For cells analyzed in A-D, n >=200; for HTM, n=500.





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