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Fig. 5. Colocalization of SRC-3 coactivator and GFP-ER at the PRL array. PRL-HeLa cells transiently expressing GFP-ER were treated with vehicle or the ligands indicated (10 nM, 2 hours). Afterwards the cells were immunostained for SRC-3. The fluorescent signal origin is labeled above the panels and merged images include DAPI-stained nuclei. n
200 for cells analyzed. The size bar indicates length in microns.