JCS03179 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Cloning of zebrafish GA2692/HspA12B. (A) Comparison of deduced amino acid sequences of zebrafish GA2692/HspA12B with human, mouse and rat HspA12B. (B) Identity matrix for HspA12Bs from zebrafish, human, mouse, rat and chimpanzee, and HspA12A (human). (C) Phylogenetic dendrogram showing the relationship of zebrafish HspA12B in different species.

• Movie 1
• Movie 2 -

  Movies 1, 2. Time-lapse analysis of morphants during sprouting of primary ISVs. 1.0 mM MOATG or MM were injected into embryos at one-cell stage and age-matched embryos were selected without prior bias as to the status of ISV sprouts at 16-somite to 18-somite stage because the embryos only had dorsal aorta and post cardinal vein laid out in the trunk. The embryos were embedded in agarose and movies were taken for 8-10 hours. Movie 1, rostral is to the right; Movie 2, rostral is to the left.