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Figure 4


Fig. 4. Loss of Pfn1 expression affects HUVEC spreading: (A) Phase contrast images of HUVECs seeded 1 hour after plating on a substrate that is pre-coated with 100 µg/ml matrigel show higher proportion of spreading cells (appear phase dense) in the control group (denoted by `C'). Impaired cell spreading was evident from round morphology of majority of Pfn1-deficient cells (denoted by `P'). Bar, 100 µm. (B) A bar graph plotting the percentage of spreading cells at two different time-points (30 minutes and 1 hour) and for two different coating concentrations of matrigel clearly shows increased spreading efficiency of the control cells (these data were pooled from the analyses of approximately 800-1000 cells for each experimental condition from two independent experiments). The asterisk indicates P<0.001. (C) Pfn1-deficient cells were still found to be much less flat and spread-out compared with the control cells at 22 hours after cell-seeding on matrigel-coated substrates. Bar, 50 µm.





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