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Fig. 1. Western blot analysis using anti-PAIR2 antibody (top), pre-immune serum as a control (middle) and anti- ${alpha}$ -tubulin monoclonal antibody (bottom) against recombinant PAIR2 protein and crude extracts from anthers and vegetative tissues. Lanes M and 1 represent a molecular weight marker and 6xHis-tagged PAIR2, respectively. Lanes 2-8 show crude extracts from anthers of the following length ranges: 0.4 $~$ 0.5 mm (2), 0.5 $~$ 0.6 mm (3), 0.6 $~$ 0.7 mm (4), 0.7 $~$ 0.8 mm (5), 0.8 $~$ 0.9 mm (6), 0.9 $~$ 1.0 mm (7), 1.0 $~$ 1.1 mm (8). Lanes 9 and 10 are from seedlings and roots, respectively.

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