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Fig. 3. PAIR2 associates with axial cores of meiotic chromosomes in wild-type (A-G) but not in pair2-null mutant PMCs (H). Panels from left to right indicate the localization of histone pan antibody (representing chromatin regions), the localization of PAIR2, and merged image of histone pan antibody (red) and PAIR2 (green). In all images, an unstained circular body within the PAIR2 signals represents the nucleolar region. (A) Pre-meiotic S/G2. (B) Filamentous PAIR2 signals begin to elongate at early leptotene. (C) Late leptotene. (D) Zygotene. (E) Pachytene. (F) Diakinesis. Pairs of arrows indicate pairs of the PAIR2 foci whose bilateral localization on bivalents is clearly detected. (G) Metaphase I. (H) Immunofluorescence against a zygotene PMC of the pair2 mutant. Bars, 5 µm.





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