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Fig. 7. PAIR2 function is not required for sister chromatid cohesion and kinetochore assembly in rice meiosis I. (A,B) Spread and Giemsa-stained meiotic chromosomes of the wild-type (A) and pair2-null mutant (B) at anaphase I. In both meiocytes, a diploid number (2n=24) of univalents was detected. (C-E) Spindle microtubules and chromosomes were stained with anti-{alpha}-tubulin antibody (green) and anti-histone pan antibody (red), respectively, in the wild-type (C) and pair2 mutant (D,E) meiocytes. Even lagging univalents at midzone were captured by spindle microtubules at mid anaphase I (two univalents showing bipolar attachment are magnified in the bottom right of D). In E, at late anaphase I, a single OsCenH3 focus attached to bipolar microtubules is elongated towards both poles (magnified in the bottom right), and a univalent shows monopolar microtubule attachment (magnified in the top left). Bars, 5 µm.





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