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Fig. 5. NAADP-induced Ca²⁺ release from both the ER and the store in the granular pole is blocked by ruthenium red and ryanodine. (A) Control trace showing that both first and second additions of NAADP trigger similar Ca²⁺ releases from the internal stores of permeabilized cells. (B) NAADP induces typical control Ca²⁺ release, but cannot release Ca²⁺ in the presence of ruthenium red. (C) Ruthenium red (10 µM) abolishes both cADPR-elicited (10 µM) and NAADP-elicited (100 nM) Ca²⁺ release. (D) IP₃ (10 µM) induces typical Ca²⁺ release response in the presence of ruthenium red. (E) NAADP induces typical control Ca²⁺ release, but in the presence of ryanodine the NAADP response is blocked. (F) cADPR induces typical control Ca²⁺ release response, but in the presence of ryanodine (100 µM) both the cADPR and NAADP responses are blocked. (G) Control trace showing that both first and second additions of cADPR induce similar Ca²⁺ releases from the internal stores of permeabilized cells. (H) IP₃ induces typical Ca²⁺ release response in the presence of ryanodine. Cells were loaded with Fluo-5N AM. All traces represent experiments on permeabilized cells. The region of interest is the whole cell in all cases.

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