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Fig. 6. Clamping [Ca²⁺] at 100 nM does not block Ca²⁺ release from ER, but blocks NAADP response from thapsigargin-insensitive Ca²⁺ store. (A) Ca²⁺ concentration was clamped (100 nM) by using a Ca²⁺/BAPTA mixture with a high concentration of BAPTA (10 mM). Both NAADP (100 nM) and IP₃ (10 µM) induced Ca²⁺ release. (B) NAADP failed to induce Ca²⁺ release after application of thapsigargin (10 µM) at clamped [Ca²⁺]. (C) IP₃ induced Ca²⁺ release after treatment with thapsigargin at clamped [Ca²⁺]. (D) cADPR (10 µM) induced Ca²⁺ release after treatment with thapsigargin at clamped [Ca²⁺]. All traces represent whole-cell regions of interest. Cells were loaded with Fluo-5N AM.

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