spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Localization of M-is6 epitopes in HeLa cells. (A) Immunoblot analysis of lysates from HeLa cells transfected with GFP-is6 and probed with immune rabbit serum 5460 against M-is6 (5460; Im), preimmune serum 5460 (PI), or immune 5460 pretreated with purified M-is6 protein (Inh). (B) Immunoblots of protein lysates of whole HeLa cells (cells) or purified HeLa nuclei (nuclei) separated on 2.5-7.5% SDS-PAGE to resolve high molecular-weight proteins. Strips were probed using serum 5460 as in (A). The 584-kDA molecular mass marker consisted of crosslinked phosphorylase B (Sigma, St Louis, MO). (C) Indirect immunofluorescence localization of endogenous titin M-is6 epitopes in HeLa cells. Cells were triple-stained with immune rabbit serum 5460 against M-is6 (red, Cy3), or serum 5460 preincubated with purified M-is6 antigen (Im + Ag), or pre-immune serum 5460 (PI) and counter-stained using antibodies against lamin A (green; FITC) and DAPI to stain DNA. (D) Indirect immunofluorescence staining of isolated HeLa cell nuclei using the same antibodies as in (A). Arrows indicate three of many puncta in which lamin A and titin potentially colocalized. Bars, 2 µm.





Right arrow Return to article