spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. (A) Schematic diagram of C. elegans titin protein (Ce-titin) showing the PEVT domain and polypeptide antigens used to raise antibodies EU145, EU102 and EU143 (grey boxes). (B) Adult C. elegans stained by indirect immunofluorescence for endogenous Ce-titin using affinity-purified EU102, EU145 and EU143 antibodies. All three gave typical striated staining in the muscle myofilament lattice. (C) Indirect immunofluorescence staining for endogenous Ce-titin (R, red; Cy3) and Ce-lamin (G, green; FITC) in wild-type embryos stained with affinity-purified EU145 or EU143 antibodies. DNA was stained with DAPI. Ce-lamin was detected with rabbit serum 3932 (Gruenbaum et al., 2002). (D) Indirect immunofluorescence staining for endogenous Ce-titin using rabbit polyclonal EU102 (G, green; FITC) and Ce-lamin rat serum 3933 (R, red; Cy3). DNA was stained with DAPI in wild-type (WT) or lmn-1(RNAi) embryos. Bars, 5 µm.





Right arrow Return to article