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Fig. 1. siRNA-mediated suppression of Beclin expression in U-251 glioma cells. U-251 cells infected with retroviral vectors and surviving after 6 days in medium containing 1 µg/ml puromycin were pooled to generate stable cell lines. Expression levels of Beclin, LAMP1 and LDH were determined in whole-cell lysates from control and Beclin KD cells by immunoblot analysis as described in the Materials and Methods.





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