spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Immunoprecipitated Beclin complex from U-251 cells contains hVps34 but not Bcl-2 or Bcl-XL. (A) Cell lysates were prepared from parallel cultures as described in the Materials and Methods and 10% of each lysate was saved for immunoblot analysis. The remainder of each lysate was immunoprecipitated with either IgG against Beclin or a `control' IgG against an unrelated protein (SPARC, Santa Cruz Biotechnology). Equal aliquots of the immune complexes eluted from protein A Sepharose beads were probed by immunoblot analysis using the antibodies indicated at the left of each panel. The band appearing just below Beclin in the upper panel is a nonspecific crossreacting protein. (B) Immunoprecipitation was performed as described in panel A, except that a rabbit antibody against Bcl-2 was used for the pull-down. The immunoprecipitated protein complexes were probed with the antibodies indicated at the left of each panel.





Right arrow Return to article