spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Suppression of Beclin expression impairs macroautophagy as measured by sequestration of LDH. Control or Beclin KD cells were incubated in DMEM + 10% FBS (normal) or HBSS (starved) for 4 hours. Cells were lysed and fractionated as described in the Materials and Methods. LDH was assayed by immunoblotting aliquots of the cell lysate, and the pellet fraction was recovered after centrifugation through a metrizamide/sucrose cushion. LDH sequestered in the pellet fraction was expressed as a ratio to the total LDH in the cell lysate. The results are derived from separate fractionations of cells from three parallel cultures (mean ± s.e.).





Right arrow Return to article