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Figure 1


Fig. 1. Invasive tumor formation after in vivo transplantation was dependent upon loss of E-cadherin. (A-C) Clinical appearance of 3D surface transplants (left panel): 3D tissues were engineered in vitro as 4:1 mixtures of HEK and either pBabe-, H-2kd-EcadC25- or H-2kd-Ecad-II-4-expressing cells and transplanted to the dorsum of nude mice for 4 weeks. Grafts harboring E-cadherin-deficient, H-2kd-Ecad-expressing II-4 cells generated nodular tumors with focal areas of erythema (C, arrow). By contrast, grafts containing II-4-pBabe (A) or H-2kd-EcadC25-expressing cells (B) generated normal grafts without any evidence of tumor formation. (D-F) Histological appearance of 3D surface transplants. Tumors generated in transplants of 4:1 mixtures of HEK and H2kd-Ecad-expressing II-4 cells demonstrated invasion of individual cells (F, inset) and small clusters of poorly differentiated tumor cells (F). By contrast, grafted mixtures of HEK with either pBabe- (D) or H-2kd-EcadC25-expressing II-4 cells (E) generated normal epithelia without any evidence of residual tumor cells. Bars, 20 µm.





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