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Fig. 1. (A) A schematic diagram indicating the location of mutations within the cdc37^ts mutant alleles cdc37-681 (Leu285 to Pro), cdc37-184 (Ala287 to Asp), cdc37-13 (Glu237 to Lys and Tyr261 to His) and cdc37-J (Leu286 to Met, His305 to Leu and Arg314 to Gly). Alignment of the human and S. pombe Cdc37 protein sequences enabled the Hsp90-binding domains (Roe et al., 2004) (horizontal stripes) and the homodimerisation domain (Roe et al., 2004) (diagonal stripes) to be mapped from the human to the S. pombe protein. (B) The cdc37^ts mutants and the cdc37⁺ strain ED1022 were streaked on YE plates and incubated at 28, 32 and 36°C for 4 days to examine the ability of each strain to form single colonies at different temperatures

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