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Fig. 2. (A) Analysis of cell number of cdc37-184, cdc37-681 and cdc37⁺ ED1022 strains. Strains were cultured at 28 and 36°C over an 8 hour time course, and samples taken at 2 hour intervals for determination of cell number using a Coulter electronic particle counter. (B) Comparison of Cdc37 protein levels in cdc37^ts mutants and the cdc37⁺ strain ED1022 after 8 hours at 28 and 36°C. Western blot analysis was carried out on whole-cell protein extracts using the anti-S. pombe Cdc37 antibody. ß-tubulin was detected by TAT1 antibody and used as a loading control. (C) Cell morphology of cdc37^ts mutants cdc37-184 and cdc37-681 and the cdc37⁺ strain ED1022 on YE plates incubated at 28 and 36°C for 24 hours. Bar, 10 µm. (D) Mean cell length of cdc37-184, cdc37-J, cdc2-33 and cdc2-L7 and cdc37⁺ cells (with s.d. bars). Strains were cultured in liquid YE at 28 and 36°C over an 8 hour time course and the lengths of 200 cells measured for each sample.

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