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Fig. 1. Cytosolic release of TCA-insoluble CFTR fragments. CFTR degradation was carried out in the absence of inhibitors (A), or in the presence of 100 µM MG132 (B), 100 µM clasto-lactacystin ß-lactone, 500 µM GPFL and 100 µM leupeptin (C), 100 µM clasto-lactacystin ß-lactone (D), 100 µM ALLN (E), 40 µM hemin (F) or hexokinase and 2-deoxyglucose (G) as described in Materials and Methods. Total (
) and TCA-soluble (
) CFTR radioactive fragments recovered in the cytosolic fraction are expressed as the percentage of total CFTR counts present at t=0. The difference between total and TCA-soluble counts released (double arrow) indicates relative amount of CFTR released in the form of TCA-insoluble fragments. The gray line (panels B-G) shows total CFTR converted into TCA-soluble counts under control conditions.
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