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Fig. 3. Released CFTR fragments are heterogeneous. (A) Supernatants of CFTR-degradation reactions were collected at the times indicated and analyzed by SDS-PAGE (7-12% gel) and autoradiography. Inhibitor concentrations were 100 µM for MG132, ALLN, clasto-lactacystin ß-lactone (ß lactone) and 40 µM for hemin. Hexokinase and 2-deoxyglucose were added to deplete ATP (–ATP). (B) Total products of the degradation reaction are shown to demonstrate the rate of disappearance of CFTR protein. High Mr material in lanes 26-30 (hemin treatment) represents ubiquitylated CFTR products (Xiong et al., 1999). Exposure of the top panels was longer (5x) than the bottom panels.





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