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Fig. 2. CD24 alters SDF-1-induced chemotaxis in pre-B cell lines. (A) Cytofluorographic analysis of N232.18, 18H18– (CD24–/–) and 18H18+ (CD24+/+) cells with monoclonal antibodies to CD24 (mAb 79) followed by PE-conjugated goat anti-rat IgG and chemokine receptor CXCR4 with biotinylated mAb 2B11 followed by streptavidin-PE. (B) Chemotactic cell migration of N232.18 and 18H18– (CD24–/–) versus 18H18+ (CD24+/+) cells in response to SDF-1. *P≤0.05; **P≤0.03. (C) Chemotactic cell migration of N232.18 (CD24–/–) versus 18H18+ (CD24+/+) towards chemokines IP-10, MIP-3{alpha}, BLC and SDF-1. *P≤0.033. (D) Detection of CXCR4 receptor by western blot analysis. Cell lysates from the indicated cell lines were tested. Calnexin was detected as a loading control. (E) Binding of biotinylated SDF-1 to CD24–/– (N232.18 and 18H18–) and CD24+/+ (18H18+) cells. Cells were stained with biotinylated SDF-1 followed by streptavidin-FITC and analysed by FACS.





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